Molecular Phylogenetic Studies of Clithon retropictus Using Arginine Kinase Gene Sequence
Chan Eui Hong, Min Kyu Sang, Dae Kown Song, Jie Eun Park, Yong Tae Kim, Hyun Jun Sin, Liu Ziwei, Hee Ju Hwang, So Young Park, Se Won Kang, Jun Sang Lee, Ki Yoon Jung, Yong Seok Lee
Department of Biology, College of Natural Sciences, Soonchunhyang University, Asan, Chungnam 8, Korea Department of Biological science, Daegu University, Gyeongsan 8, Korea Korea Native Animal Resources Utilization Convergence Research Institute Perf
Clithon retropictus is a small gastropod belonging to the Neritidae, and inhabits gravel areas in brackish waters. It has been designated as an endangered species as class II, because of its habitat conditions and reckless development of coastlines. In September 2021, C. retropictus registered in NCBI has only 80 nucleotides, 52 proteins, and 74 genes. In this context, we sequenced the C. retropictus cDNA library using the Illumina Hiseq 2500 platform. Arginine Kinase (AK) sequence was obtained by using BLAST program. To investigate the molecular phylogenetic analysis of C. retropictus, a dendrogram was generated using AK gene as a marker. Based on the phylogenetic tree, it is classified into three classes (Cephalopods, Gastropods, and Bivalvia). In addition, analysis of AK 2D structure using 38 species shows that no striking similarity was found. In conclusion, AK gene of C. retropictus consisted of 1,053 bp (351 amino acids) and could be utilized for molecular phylogenetic classification.
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