Classification and Phylogenetic Studies of Cephalopods from four countries of South-East Asia
Hee Ju Hwang, Se Won Kang, So Young Park, Jong Min Chung, Dae Kwon Song, Hyeongchun Park, Hong Seog Park, Yeon Soo Han, Jun-Sang Lee and Yong Seok Lee
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, Asan, Chungnam 31538, Korea Department of Chinese Language and Literature, College of Humanities, Soonchunhyang University, Asan, Chungnam 31538, Korea
In this study, an attempt has been made to analyze the morphology of Cephalopods distributed in Korea and collected samples from South-East Asian countries including Thailand, Indonesia, Vietnam, and China. A phylogenetic analysis was performed using the mitochondrial gene, Cytochrome c oxidase subunit I (COI) to understand the genetic divergences of the species and validate their origins. For achieving the objectives, samples were collected directly from Thailand Hat Yai, Songkhla, Indonesia Medan, Vietnam Ho Chi Minh, and Vung Tau in August 2015 and from China in September 2015. A total of 23 species of Cephalopods were identified  falling under three orders, four familyies and nine genus. The species were distributed under Order: Octopoda (1 family, 3 genus, and 9 species), Order: Sepiolioda (1 family, 2 genus, and 8 species), and Order Teuthoidea (2 family, 4 genus, and 6 species). 23 species which is 1 family 3 genus 9 species in Octopoda, 1 family 2 genus 8 species in Sepiolioda, 2 family 4 genus 6 species in Teuthoidea. Phylogenetic analysis using COI gene was conducted for 18 species. For the remaining 5 species sequencing results showed severe variation and hence were not considered further. The COI phylogenetic analysis for the 18 species of Cephalopods were found consistent with the morphological identification. The excluded species will be subjected for a further detailed analysis.
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