Phylogenetic Analysis based on Metallothionein Gene Sequence of an Indigenous Species Pisidium (Neopisidium) coreanum in Korea
Moon Ki Baek, Jun-Seo Lee, Se Won Kang, Jae Bong Lee, 1Hyun Jung Kang 2Yong-Hun Jo, Mi-Young Noh, 2Yeon Soo Han, 3Sang-Haeng Choi, 4Sung-Hwa Chae, 3Hong-Seog Park, 5Jun-Sang Lee and Yong Seok Lee
Department of Parasitology, Inje University College of Medicine, Busan 614-735, Korea 1Fisheries Resource Research Department, National Fisheries Research and Development Institute, Busan 619-902, Korea 2Department of Agricultural Biology, Chonnam Natio
Pisidium (Neopisidium) coreanum is a freshwater snail and lives in spring water near mountain areas. Interestingly, this snail has traditionally regarded as medicinal food, and thus has been used as folk remedies for healing broken bones. Recently, alpha classification on Pisidium (Neopisidium) coreanum through re-descript-xion has been conducted. However, not much attention has so far been made in beta classification. In this study, we performed the beta classification based on metallothionein (MT) genes found from various organisms. To this end, the complete cDNA sequences were obtained from the Expressed Sequence Tag (EST) sequencing project of Pisidium (Neopisidium) coreanum. The coding region (315 bp) gives an amino acid sequence of 105 residues. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of Pc-MT gene indicate that Pisidium (Neopisidium) coreanum has similarity to freshwater bivalve such as Dreissena polymorpha (zebra mussel), Unio tumidus (swollen river mussel) and Crassostrea ariakensis (suminoe oyster).
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