Molecular Phylogenetic Analysis of Koreanomelania nodifila using Arginine Kinase gene
Hyeok Lee, Min kyu Sang, Dae kwon Song, Jie Eun Park, Jun Yang Jeong, Chan Eui Hong, Yong Tae Kim, Hyun Jun Sin, Yi Seon Jang, Hee-Ju Hwang, Se Won Kang, So Young Park, Hong Seog Park, Jun Sang Lee, Yeon soo Han8 and Yong Seok Lee,
Department of Biology, College of Natural Sciences, Soonchunhyang University, Asan, Chungnam 31538, Korea, Korea Native Animal Resources Utilization Convergence Research Institute (KNAR), Soonchunhyang University, Asan, Chungnam, Korea, Research Support

Arginine kinases (AK) are known to be widely distributed in various invertebrates. It has an essential role in cellular energy metabolism. It regulates energy regulation and homeostasis in response to external stress. AK gene is used as a marker in invertebrate phylogenetic analysis. In this study, we verified whether AK can be used in molecular phylogenetic analysis. We sequenced Koreanomelania nodifila cDNA library using Illumina Hiseq 2000. The AK gene of K. nodifila was sequenced using the BLASTx program and PANM DB (v5.1). AK gene was used as a marker to molecular phylogenetic using the MEGA 11 maximum likelihood method. To analyze the 2D structure of the AK gene, the 2D structure was predicted using PSIPRED (v4.0). As a result, the AK gene ORF of K. nodifila is a total of 352 amino acids and AK gene is considered to be highly useful for molecular phylogenetic analysis of Mollusca.

  
39-4-11-199-205.pdf (1.7M), Down : 11, 2024-01-16 16:06:05

 

사무국 & 편집국 : 충남 아산시 신창면 순천향로 22 자연과학대학 3317호 / Tel: 041-530-3040 / E-mail : malacol@naver.com