Verification method of triploid abalone, Haliotis discus hannai using genetic marker
Hyejin Kim and Choul-Ji Park
Fisheries Seed and Breeding Research Institute, NIFS, Haenam, 59002, Korea
The method for ploidy verifications is necessary for efficient research and development of triploid abalone, Haliotis discus hannai. The various methods such as flow cytometry, karyotyping, and particle size analysis have been used to confirm ploidy. However, this method provides only an approximate estimation of the ploidy level and measuring cellular DNA content is not only time consuming, but also costly and labor intensive. In this study, a quick and accurate method for ploidy verification of triploid and diploid abalone, H. discus hannai, was developed using multiplex polymerase chain reaction (PCR) system for molecular microsatellite markers. The multiplex PCR system including five microsatellites markers from Pacific abalone, H. discus hannai, consisting of di, tri, tetra and pentanucleotide repeat units, is developed. The multiplex PCR was able to verify the ploidy of 14 triploid abalone with 100% accuracy, In this results suggest that multiplex PCR system may be a useful tool in verification of triploid abalone.
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