Characteristics of growth about addition of δ-aminolevulinic acid with recombinant Escherichia coli at Isochrysis galbana and Chlorella sp.
Si-Woo Lee, Bo Gyeong Seol, Choon-Sup Kim, Wi-Sik Kim
Southeast Sea Fisheries Research Institute, NIFS, Tongyeong 53085, Korea EnbioGene, Yeosu 5977, Korea Department of Aqualife Medicine, Chonnam National University, Yeosu 5966, Korea
In this study, δ-aminolevulinic acid (δ-ALA), a plant growth promoter, production with E. coli recombined with microalgae Isochrysis galbana and Chlorella sp. was added without (control), f/2 medium, δ-ALA 20 ppm, f/2 medium +δ-ALA 1, 10, 20 ppm was added and cultured for 12 days to investigate the number of cells and the specific growth rate (SGR). In I. galbana, a haptophytes, both the cell number and SGR were significantly higher in the experimental group cultured with f/2 medium at 2, 3, 5 day (P < 0.05). In the δ-ALA added group, f/2A10 with 10 ppm of δ-ALA added to f/2 medium was significantly higher than in all groups expect f/2A20 at 5 day (P < 0.05). In Chlorella sp., a chlorophyta, the cell number and SGR of f/2A1 were significantly higher than those of all groups except f/2A20 (it was excluded from the culture due to a test error) at 1 day (P < 0.05). At 2, 3 and 5 day, all of the δ-ALA added groups showed significantly higher cell numbers and SGR than the control and f/2 groups (P < 0.05). At 8 day, f/2A1 was significantly higher than that of all the groups including the δ-ALA-added group (P < 0.05), and continued until the 12th day. Therefore, δ-ALA production with recombinant E. coli had little growth promoting effect in I. galbana, but showed a growth promoting effect in Chlorella sp..
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