Molecular Phylogenetic Study of Clithon retropictus using metallothionein gene
Jun Yang Jeong, Min Kyu Sang, Jie Eun Park, Dae Kwon Song, Chan-Eui Hong, Yong Tae Kim, Hyun Jun Sin, Hee Ju Hwang, Sang-Mok Jung, So Young Park, Se Won Kang, Jun Sang Lee, Yeon Soo Han, Hong Seog Park, Yong Seok Lee, and Wan-Jong Kim
Department of Biology, College of Natural Sciences, Soonchunhyang University, Asan, Chungnam 31538, Korea Department of Biological science, Daegu University, Gyeongsan 38453, Korea Korea Native Animal Resources Utilization Convergence Research Institute
Clithon retropictus is a small gastropod belonging to Neritidae that lives in gravel areas of the brackish water zone. Due to these limited habitat conditions and indiscriminate coastal development, it has been designated as a second-class endangered species since 2005. Although its continued interest is low, it is important to investigate the endangered species-specific characterization and analysis of its genetic information. As of June 2021, genetic information of C. retropictus registered in the NCBI was 78 cases, and related studies are also insufficient. In addition, it has not yet been studied the identification and functional characterization of MT gene, which plays a critical role in controlling metal toxicity and oxidative stress, in C. retropictus. In this context, we analyzed basic structure and specific domains of MT sequence of C. retropictus (CrMT) and the taxonomic location of CrMT with its othologs registered in NCBI. As a result, CrMT gene containing with mollusca-specific MT pattern was identified in C. retropictus. In addition, phylogenetic analysis through the maximum-likelihood method indicates that C. retropictus was closely related to the order Lepetelida inhabiting the sea. Taken together, our results may support basic information of endangered species living in brackish water zone.
  
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