Larval breeding using Bacillus species, an intestinal microorganism isolated from Surf Clam (Tresus keenae)
Han Seung Kang, Hee Chan Choi, Jung Hyun Cho and Chul Won Kim
MS BioLab, 3F, 103 Gayang-ro, Dong-gu, Daejeon 34576, Korea Fisheries Resources and Environmental Division, East Sea Fisheries Research Institute, Gangneung 25435, Korea Department of Aquaculture, Korea National College of Agriculture and Fisheries, Kon
In this study, we conducted a larval breeding study of Tresus keenae using 5 Bacillus genera, which are excellent in the ability to decompose high molecular weight organic matter and the antibacterial activity of pathogenic bacteria, among the 65 Bacillus genera. Research results that the relationship between larval growth and viability was high in the TKI42 and microbial mixed strains of TKI02 + TKI14 + TKI26 + TKI32 + TKI42. The growth and viability of larvae at the amount of the mixed microbial strain (TKI02 + TKI14 + TKI26 + TKI32 + TKI42) was 1.0×106 CFU/ml, which was the best growth, and 1.0×107 CFU/ml, which was the lowest. And the survival rate was the best at 1.0×105 CFU/ml. In addition, the growth and survival rate were high when the mixed microbial strain (1.0×106 CFU/ml) with a water temperature of 25°C was treated. In this study, when the growth and survival of larval breeding was investigated based on five Bacillus strains, the prediction of the application to the breeding of organisms in the aquaculture industry based on the special ability of a single strain is uncertain. Rather than developing and using only a single strain, it is thought that the effect can be expected to using mixed strains.
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