In this study, 30 polychaete samples were isolated from abalone shells, and genetic classification was performed using them. The DNA sequences corresponding to the polychaete 18S rDNA and 16S rDNA or mitochondrial cytochrome c oxidase I (CO1) regions were decoded, and the types were identified through BLAST search of NCBI. As a result of confirming the nucleotide sequence, genetic diversity was analyzed for 27 samples that were translated into ‘Annelida’, and 16 samples were identified as Polydora hoplura. In addition, Annelids such as Amphisamytha vanuatuensis, Amythas membranifera, Cirriformia chicoi, Cirratulus cirratus, Hediste spp., Notophyllum japonicum, Phyllodoce rosea, Syllis armillaris, and Terebella lapidaria have been identified. In the future, it is expected that various studies on several types of Annelids identified in abalone farms as well as continuous research on P. hoplura are needed.