Molecular Phylogenetics Analysis of geoduck (Panopea japonica) apply Metallothionein gene
Dae Kwon Song, Hee-Ju Hwang, Jong Min Chung, Jie Eun Park, Min Kyu Sang, Jun Yang Jeong, Se Won Kang, Myung-Mo Nam, Jong Dae Lee, Hong seog Park, Yong Hun Jo, Jun Sang Lee, So Young Park and Yong Seok Lee
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, Asan, Chungnam, 31538, Korea
Metallothionein (MT) plays an important role in protecting cells from harmful substances. MT is a protein with less than seven kDa with large amounts of cysteine residues, which have been studied as a phylogenetic biomarker with unique patterns not only in vertebrates but also in Mollusca. Panopea japonica is a giant clam that lives on the coast of the East Sea in Korea. Nevertheless, little research has been done on the genetic resources of P. japonica, and the amount of genetic information registered in the NCBI is very limited. In this study, a metallothionein sequence consisting of 74 amino acids with 222 bp nucleotide was obtained from the transcript-xome data by using the GS FLX 454 platform. In Silico analysis of PjMT gene was conducted with ClustalX and MEGAX programs. With the previously reported Mollusca MT data, we verified the PjMT sequence as a potential candidate for molecular marker, and also confirmed that PjMT was clustered in Myoida order.
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