Molecular phylogenetic study of bivalvia from four countries (China, Japan, Russia and Myanmar) using 3 types of primers
Jong Min Chung, Hye Rin Min, Min Kyu Sang, Jie Eun Park, Hang Chul Cho, Se Won Kang, So Young Park, Hong Seog Park, Sang Yong Park, Eun Kyung Kang, Konstantin A. Lutaenko, E Hyun Shin, Jun-Sang Lee, Yong Seok Lee and Hee Ju Hwang
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, Asan, Chungnam, 31538, Korea Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 56212,Korea Animal
Imports of aquatic products continue increasing every year. When imported into Korea, the common name of a similar aquatic product is used instead of a scientific name, causing many confusions. In this study, in order to cope up with this issue, four countries with the highest number of imported bivalves were selected. The collected bivalves were totally classified into 10 orders, 17 families, 39 species. Also, the collected bivalves in the current study were identified by morphological classification. Moreover, sequence analysis of COI gene was carried out for all the collected species. The COI gene sequence was obtained with 3 type of primers. All COI sequences analyzed were subjected to molecular phylogenetic analysis. As a result, it was possible to draw conclusions very similar to the morphological classification results. Taken together, it is preferable to perform the universal primers and the lobo primer simultaneously for analyzing the COI sequence of bivalves. On the other hands, to obtain the results for scallops, the Matsumoto primers were available to conduct the analysis.
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