Molecular Phylogenetic Analysis based on Metallothionein Gene Sequence of an Endangered Species Cristaria plicata in Korea
Jong Min Chung , Hee-Ju Hwang, Min Kyu Sang, Jie Eun Park, Hye Rin Min, So Young Park, Young-Su Park, Mi Young Noh, Yong Hun Jo, Yeon Soo Han, Jun Sang Lee, Seung-Hwan Park, Se Won Kang, Chang Soo Kang and Yong Seok Lee
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, Asan, Chungnam, 31538, Korea Biodiversity Conservation & Change Research Division, Nakdonggang National Institute of Biological Resources, Sangju, Gyeonb
Cristaria plicata is enlisted as an endangered wild species class Ⅰ under the Red data list of National Institute of Biological Resources, South Korea. In this study, we have attempted to do molecular phylogenetic analysis by using metallothionein (CpMT) gene of C. plicata. The complete CpMT cDNA sequences were obtained from unigenes (analyzed NGS transcript-xome data) of C. plicata. We used Illumina Hi-Seq 2500 next-generation sequencing platform to characterize the transcript-xome resources of C. plicata. De novo analysis using the Trinity program and TGICL clustering resulted in the number of 374,794 (276,264,683 bp) unigenes. CpMT sequences were 210 bp coding for a protein 70 amino acid residues using sixpack (EMBOSS package) and genscan software. Multiple sequence alignment and phylogenetic analysis were performed by MEGA7 software. Molecular phylogenetic analysis indicates that CpMT has similarity with various mollusks such as Hyriopsis cumingii and Unio tumidus (Order Unionoida).
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