Effects of different diluents and cryoprotective agents on the sperm cryopreservation of ablalone (Haliotis discus hannai)
Sungwon Kang, Jae Geun Lim, Seung Cheon Seon, Kyeong Sig Lee, Jiheon Kim, Tae Hyug Jeong, Young Dae Oh and Han Kyu Lim
Jeollanam-do Ocean and Fisheries Science Institute Department of marine fisheries resources, Mokpo National University
The purpose of this study was to examine various cryoprotective agents (CPAs) and diluents on post-thaw motility and fertility of Haliotis discus hannai sperm based on movable sperm ratio (MSR), sperm activity index (SAI), fertilization and hatching rate. To evaluate the optimal CPA for cryopreservation of H. discus hannai sperm, the sperm was diluted in three extenders (sea water filtered, 1 M glucose, 1 M, sucrose) containing one of three CPAs (dimethty sulfoxide [DMSO], glycerol [G], ethylene glycol [EG]) at the concentration of 1, 2.5, 5, 10, 20%, and then frozen in programmable freezer. Sperm diluted with sea water containing 2.5% DMSO had the best post-thaw MSR (40.0 ± 2.9%), SAI (3.3 ± 0.1%), fertilization rate (50.8 ± 15.0%) and hatching rate (76.5 ± 4.5%).
  
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