We investigated the survival rate by water temperature and salinity, physiological rhythm and morphological change of live abalone to get to know optimum water temperature and salinity suitable for long-distance transportation of live abalone. At 8°C and above, 96-100% of survival rate was shown at all experiment groups. At 6°C, 66% of abalones survived in normal seawater but they showed 0% of survival rate at 30 ± 0.5 psu and 26 ± 0.5 psu of salinity at the same water temperature. There was no significant difference of oxygen consumption rate for a week between the seawater and 30 ± 0.5 psu. Also, a positive correlation was shown between salinity and water temperature and the oxygen consumption rate was slightly higher at 30 ± 0.5 psu than seawater. Thinned epithelial layers and expansion of lymph sinus were observed less than 30 ± 0.5 psu or below 6°C of temperature. This result shows that the optimum level of water temperature and salinity is considered to be 6-8°C and more than 30 ± 0.5 psu respectively.