Analysis of genetic divergence according to each mitochondrial DNA region of Haliotis discus hannai

Choul-Ji Park, Won Sick Nam, Jeong-Ho Lee, Jae Koo Noh, Hyun Chul Kim, Jong won Park, In Jun Hwang and Sung Yeon Kim
Genetics and Breeding Research Center, NFRDI, Gyeongsangnam-do 656-842, Korea
The seven mitochondrial DNA regions (ND2, ND5, ND4, ND4L, ND6, ND1 and 12SrRNA) of Haliotis discus hannai were examined to estimate the availability as a genetic marker for the study of population genetic. The region with the highest genetic variation was ND4 (Haplotype diversity = 1.0000, Nucleotide diversity = 0.0108). On the other hand, ND2 and ND1 regions have significantly appeared genetic divergence between clusters (divergence of 90% and 87%). Also, pairwise FST between clusters within ND2 and ND1 regions showed high values; 0.4061 (P = 0.0000), 0.4805 (P = 0.0000) respectively. Therefore we can infer that it is the most efficient and accurate way to analyze the region of ND4 with the highest variation in addition to the regions of ND2 and ND1, which formed clusters with high bootstrap value, for study of population genetic structure in this species.
  
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