전자저널 > 전자저널 > Maturity and Spawning of the Triploid Pacific Abalone, Haliotis discus hannai

Maturity and Spawning of the Triploid Pacific Abalone, Haliotis discus hannai

Young Ju Jee, Bo Hye Nam, Jeong Yong Lee and Young Jin Chang

Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang-gun, Busan 619-705, Korea Research and Planning Division, National Fisheries Research and Development Institute, Gijang-gun, Busan 619-705, Korea Department o

The gonadal development of triploid and diploid Pacific abalones, Haliotis discus hannai was histologically investigated in spawning season. Diploid abalones had matured oocytes and spermatozoa, but most triploid had spermatocytes or developing oocytes that was slightly retarded in gonadal development compared to diploid abalones. In spawning experiment of triploid and diploid abalones, spawning rates of diploid male and female were 100%, but those of triploid female was 50% and male was 25% respectively. Investigation of spawned abalone eggs and spermatozoa revealed that length of diploid sperms head were 17.47 μm, breadth of head were 10.31 μm, length of spermatozoa were 130.72 μm, but those of triploid spermatozoa were 11.83 μm, 7.89 μm and 103.36 μm respectively. Triploid spermatozoa were significantly small to diploid spermatozoa (p < 0.05). The eggs of diploid and triploid were not different in size. The cross experiment between oocytes produced by triploids and spermatozoa by diploids (3n × 2n cross) revealed that no fertilization were occurred, and 2n × 3n cross also revealed same result.

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