Molecular Phylogenetic Study of Nesiohelix samarangae Based on Metallothionein Gene
Jun-Seo Lee, Byung-Jun Min, Se Won Kang, Jae Bong Lee, Moon Ki Baek, Seung Young Hwang, So Hee Kim, Weon-Gyu Kho, Sang-Haeng Choi, Sung-Hwa Chae, Hong-Seog Park, Yeon Soo Han, Jun-Sang Lee, Kye-Heon Jeong and Yong Seok Lee
Department of Parasitology, Inje University College of Medicine and Department of Malariology, PICR, Busan 614-735, Korea
Numerous morphological studies on N. samarangae have been well conducted over the last ten years. In this context, we have attemtped to do molecular phylogenetic analysis by using metallothionein (MT) gene from N. samarangae. To this end, we cloned the full length cDNA of MT from cDNA library of N. samarangae. The complete cDNA sequences were obtained from the expressed sequence tag (EST) sequencing project of N. samarangae. The coding region of 195 bp gives an amino acid sequence of 65 residues including methionine. There are 5' (61 bp) and 3' (48 bp) untranslated region at both ends of the Ns-MT cDNA sequence. The combined results from BLAST analyses, multiple sequence alignment and molecular phylogenetic study of Ns-MT cDNA indicate that N. samarangae has similarity to land snails such as Helix pomatia, Helix aspersa and Arianta arbustorum.
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