전자저널 > 전자저널 > Comparative Study on the Salivary Gland between Two Species (Achatina fulica and Incilaria fruhstorferi) of the Snails in Stylommatophora (Mollusca, Gastropoda)

Comparative Study on the Salivary Gland between Two Species (Achatina fulica and Incilaria fruhstorferi) of the Snails in Stylommatophora (Mollusca, Gastropoda)

Jong Min Han and Nam Sub Chang

Department of Biology, College of Science and Engeneering, Mokwon University. 24 Mok-dong, Chung-gu, Taejon 301-729, Korea

Histochemical experiment was carry out respectively to confirm the properties of the salivary gland and salivary secretory ducts between two species of snails (Achatina fulica and Incilaria fruhstorferi). SDS-PAGE was carried out to compare and investigate the distribution aspects of protein patterns between the two species. Five types(A, B, F, H and I) of gland cells with four neutral mucopolysaccharide cells and one acid mucopolysaccharide cell were observed in acinous of Achatina fulica, while six types were observed in acinous of Incilaria fruhstorferi: one acid mucopolysaccharide cell(type-A) and four neutral mucopolysaccharide cells(type-B, C, D and F) and one cell that acid mucopolysaccharide is only membrane that surrounded granule(type-E). The results are follows: The type-A gland cell is commonly observed between the two species. The type-A gland cell in Achatina fulica possesses a nucleus with a developed heterochromatin, and the cytoplasm was filled with round granules. The granules were surrounded with an uncertain boundary membrane and confirmed with neutral mucopolysaccharides, but is confirmed acid mucopolysaccharide in Incilaria fruhstorferi. The type-B gland cell is observed in the two species, too. The type-B gland cell in Achatina fulica was round shaped, and included an evenly large nucleus. The nucleoplasm was a well developed heterochromatin, and was observed in nucleolus. In the cytoplasm included granules that were confirmed in the neutral mucopolysaccharides of the two species. The type-C and D gland cells exist only in Incilaria fruhstorferi, nucleoplasm was well developed heterochromatins. The type-E gland cell appears in the acinous surrounded the salivary gland of Incilaria fruhstorferi. These granules appear irregular shape and size and the cytoplasm is formed in alveolar. The type-F gland cells are commonly observed in the salivary glands of the two species. They are similar with the type-B gland cell, but the granular shape is comparatively small and irregular, and possess the neutral mucous granules. The type-H gland cells are mainly seen in only Achatina fulica, and in nucleus is a well developed heterochromatin. The cytoplasm is filled with round small granules with acid mucopolysaccharide for alcianophilia observed. The type-I cell was small cell with an irregular shape and only observed in the gland cells of Achatina fulica. The heterochromatins were developed in the nucleus and the granules are not observed in cytoplasm. Secretory ducts of saliva are composed of the interlobular duct and interlobar secretory duct. In Achatina fulica the interlobular duct consists of a simple cuboidal epithelium, while the endothelium of intralobar secretory duct consists membrane with no clear cell boundary. The interlobar secretory duct of Incilaria fruhstorferi consists of a simple squamous epithelium and in the cytoplasm is filled with granules (type-G secretory cell). A SDS-PAGE was carried out to confirm that the protein band pattern consist of salivary gland. In conclusions, five more bands in Achatina fulica and three bands in Incilaria fruhstorferi were confirmed in MW<29 kDa. one main band coincides comparatively with both and is between 29～45 kDa. There are four main bands in Achatina fulica and two main bands in Incilaria fruhstorferi between 45～66.5 kDa respectively. The bands in Achatina fulica seem more complex than in Incilaria fruhstorferi.

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